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By T. Brzozowski
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1998a) can also reduced by administration of probiotics. Strains associated with reduction of diarrhoea include Bifidobacterium spp, B. , 2000), L. rhamnosus GG, L. acidophilus, L. , 2006). , 2003; Sullivan & Nord 2005). Inflammatory bowel disease (IBD) and irritable bowel syndrome (IBS) are other intestinal disorders that can be treated with varying degrees of success using probiotics. , 2001). IBS is typically characterized by abdominal pain, excessive flatus, variable bowel habit and bloating (Madden & Hunter, 2002).
The appropriateness of PCR for quantification of viable cells can be improved by staining the samples with DNA binding dyes prior to DNA extraction and amplification. Treatment with DNA-binding dyes and subsequent PCR analysis uses membrane integrity as the criterion in determining viability of cells. , 2009). These dyes form covalent bonds with DNA upon exposure to visible bright light and thus inhibit subsequent PCR amplification. , 2009). , 2005). Though plate counting is arduous and time consuming, no method has yet been found that completely replaces it.
Foods containing probiotics are referred to by others as functional foods. , 1998). Thus, functional foods have a physiological or psychological effect beyond basic nutritional value (Clydesdale, 1997). , 2005). , 2005). , 2002). There is an increase in use of other foods as vehicles for probiotics. This is partly due to allergenicity of some consumers to milk products. , 2007) are among these alternatives. , 2007). Recently, Aragon-Alegro et al. (2007) added probiotic chocolate mousse to the list of alternatives.