Download Cereal biotechnology by Peter C. Morris, James H. Bryce PDF

By Peter C. Morris, James H. Bryce

(Woodhead Publishing constrained) Discusses genetic amendment in cereal biotechnology, explaining the foundation for present laws and techniques for assessing hazards. additionally addresses particular functions for cereal biotechnology from using molecular organic instruments cereal construction equipment.

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Extra resources for Cereal biotechnology

Sample text

3 Agrobacterium Virulent strains of the soil bacterium Agrobacterium tumefaciens are capable of infecting a wide range of dicotyledonous plants and trees, which results in crown gall disease. The virulence capability of Agrobacterium is determined by the presence of extra-chromosomal genetic information contained on a plasmid, an autonomous circular piece of DNA. The plasmids of the various Agrobacterium strains have somewhat different characteristics. These plasmids have been manipulated by recombinant DNA techniques in such a way that new genetic information inserted into the plasmid will be transferred by the Agrobacterium to the infected plant cell without causing crown gall disease.

The literature relating to maize and rice is now so extensive that it is impossible to include reference to all published work and experimental details in this chapter. Consequently, the citation of references is limited, where possible, to the most recent and relevant reports. 2 Approaches to the transformation of maize and rice Birch3 discussed the requirements for efficient transformation systems. Essentially, the transformation protocol must be cultivar-independent, technically simple and safe to operate.

It could take up to 24 hours after the removal of the PEG and calcium for them to become round and healthy again. The protoplasts will then regenerate their cell wall and start to divide. 2 Biolistics The delivery of DNA to plant cells by means of biolistic methods has allowed the use of whole tissues as targets for transformation and therefore the first successful transformation procedures for wheat and barley. The principle of biolistics is very simple: 1. 2. 3. Gold or tungsten particles smaller than the plant cells are coated with DNA.

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